Gene Expression Analysis in Cancer using RNA-Seq

Pranava Upparlapalli • June 2025

Link to GitHub Repository

🔬 Project Summary

This project analyzes RNA-Seq gene expression data across five major cancer types: BRCA, KIRC, LUAD, COAD, and PRAD. Built in R using DESeq2, PCA, and clustering to uncover patterns, expression shifts, and potential biomarkers.

Tools: DESeq2, ggplot2, pheatmap, factoextra

📊 Key Visualizations

Sample Distribution

Sample Distribution

Heatmap Expression

Heatmap of Differential Expression

📑 Full R Markdown Report